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Flotation cell apoptosis

Apr 28, 2020

Aqueous biphasic flotation (ABF) integrates aqueous biphasic system (ABS) and solvent sublation for recovery of target ... and death of a cell by induces the apoptosis via the formation of

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  • Synthetic bichalcone TSWU-BR23 induces apoptosis of
    Synthetic bichalcone TSWU-BR23 induces apoptosis of

    Using cell-surface biotinylation and sucrose density-gradient-based membrane flotation techniques, we showed that the disruption of TSWU-BR23-induced lipid raft localization of CD95/FADD by cholesterol-depleting agent (methyl-β-cyclodextrin) was

  • Extracellular vesicle‐mediated endothelial apoptosis and
    Extracellular vesicle‐mediated endothelial apoptosis and

    Jul 03, 2021 For the IZON SEC samples, the sample was used directly without any dilution and floatation was performed for 2 min followed by washing and staining. The images were acquired using transmission electron microscopy at 100KV emission (JEM-1400, ... as well as endothelial cell apoptosis by caspase-3 staining (Li et al., 2020;

  • Identification of small extracellular vesicle subtypes in
    Identification of small extracellular vesicle subtypes in

    Feb 12, 2021 This study first separated sEV subtypes from follicular fluid using differential ultracentrifugation combined with iodixanol density gradient flotation and then evaluated their miRNA profile and effects on the proliferation and apoptosis of granulosa cells (GCs)

  • Adsorption mechanism of propyl gallate as a flotation
    Adsorption mechanism of propyl gallate as a flotation

    Mar 15, 2019 Then the frother was added and the suspension was agitated for another 1 min before introducing air bubble to the flotation cell. After 3 min of flotation, the concentrate and tailing were collected separately, filtered, dried and weighed, based on which the recovery was calculated. 3. Results and discussion3.1

  • Inhibition of Plasmodium falciparumField Isolates
    Inhibition of Plasmodium falciparumField Isolates

    Inhibition of Plasmodium falciparumField Isolates-Mediated Endothelial Cell Apoptosis by Fasudil: Therapeutic Implications for Severe Malaria Estelle S. Zang-Edou1, Ulrick Bisvigou1, Zacharie Taoufiq2, Faustin Le koulou1, Jean Bernard Le kana- Douki1, Yves Traore 3, Dominique Mazier2,4,5, Fousseyni S. Toure -Ndouo1* 1Centre International de Recherches

  • Selective Aggregation of Hydrophilic Gangue Minerals in
    Selective Aggregation of Hydrophilic Gangue Minerals in

    In a mechanical flotation cell, macro hydrodynamics is responsible for solids suspension and aggregates transport, and micro-turbulence plays an important role

  • Apoptosis induced by oxidized low density lipoprotein in
    Apoptosis induced by oxidized low density lipoprotein in

    Dec 25, 2001 In contrast, programmed cell death induced by C 2-ceramide, a widely used apoptosis-inducing agent, was not prevented by either antibody demonstrating that their inhibitory effect is specific for OxLDL-triggered apoptosis. Hence CD36-binding appears to be essential for apoptosis signaling by OxLDL

  • Modified jet flotation in oil (petroleum) emulsion/water
    Modified jet flotation in oil (petroleum) emulsion/water

    a b s t r a c t This work presents results of a rapid emulsified oil (petroleum) removal from water by flocculation followed by flotation in a modified jet (Jameson) cell (MJC). The modification

  • Regulation of Fas-Mediated Apoptosis in Neutrophils after
    Regulation of Fas-Mediated Apoptosis in Neutrophils after

    As immune cells, WBC subsets undergo changes in their proportion in peripheral blood by inhibition of apoptosis of neutrophils (1)(2)(3)(4) and apoptosis of lymphocytes (7,8) in certain

  • Inhibition of Plasmodium falciparum Field Isolates
    Inhibition of Plasmodium falciparum Field Isolates

    Oct 07, 2010 Plasmodium falciparum infection can abruptly progress to severe malaria, a life-threatening complication resulting from sequestration of parasitized red blood cells (PRBC) in the microvasculature of various organs such as the brain and lungs. PRBC adhesion can induce endothelial cell (EC) activation and apoptosis, thereby disrupting the blood-brain barrier

  • (PDF) ApoE genotype-specific inhibition of apoptosis
    (PDF) ApoE genotype-specific inhibition of apoptosis

    In contrast, other fac- different APOE genotypes differentially inhibit apoptosis. tors, such as nitric oxide, estrogen, and insulin, inhibit en- dothelial cell apoptosis and are antiatherosclerotic (4–6). HDLs are also antiatherosclerotic, and have recently been MATERIALS AND METHODS demonstrated to inhibit endothelial cell apoptosis

  • Frontiers | Haemonchus contortus Transthyretin-Like
    Frontiers | Haemonchus contortus Transthyretin-Like

    In addition to the increased number of apoptotic germ cells, lower transcriptional level of Ce-ttr-31 and Hc-ttr-31 was linked to a significant increase of general ROS (p 0.01) and transcriptional level of ced-4 (cell death abnormal 4, encodes a key component in the apoptosis activation pathway) (p 0.001) in RNAi-treated worms , suggesting

  • Simvastatin induces apoptosis of Epstein–Barr virus (EBV
    Simvastatin induces apoptosis of Epstein–Barr virus (EBV

    Simvastatin induces apoptosis of Epstein–Barr virus (EBV)-transformed lymphoblastoid cell lines and ... cells. Detergent extraction and flotation assay for lipid rafts were performed as described (27). Immunoblotting was per- ... Simvastatin inhibits clump formation and induces apoptosis in LCLs. (A) Cell clumping of LCLs was observed after

  • (PDF) Inhibition of Plasmodium falciparum Field Isolates
    (PDF) Inhibition of Plasmodium falciparum Field Isolates

    Inhibition of Plasmodium falciparum Field Isolates- Mediated Endothelial Cell Apoptosis by Fasudil: Therapeutic Implications for Severe Malaria Estelle S. Zang-Edou1, Ulrick Bisvigou1, Zacharie Taoufiq2, Faustin Le koulou1, Jean Bernard Le kana- Douki1, Yves Traore 3, Dominique Mazier2,4,5, Fousseyni S. Toure -Ndouo1* 1 Centre International de Recherches

  • Cell Removal - an overview | ScienceDirect Topics
    Cell Removal - an overview | ScienceDirect Topics

    2 Apoptosis and cfDNA. The term apoptosis was first described in 1972 to characterize the dead cell morphology, although certain components, descriptions, and processes of cell death have been previously described [72–74]. Since then, the term apoptosis has been adopted and has been used to describe the mechanism involving intrinsically

  • A Cardinal Role for Cathepsin D in Co-Ordinating the Host
    A Cardinal Role for Cathepsin D in Co-Ordinating the Host

    Jan 27, 2011 Author Summary Tissue macrophages frequently undergo a program of cell death, termed apoptosis, following sustained ingestion and killing of bacteria. In macrophages, induction of apoptosis enhances bacterial killing when macrophages' initial killing capacity is exhausted. We have investigated the mechanism of apoptosis in macrophages exposed to pneumococci

  • Lipid raft‐mediated Fas/CD95 apoptotic signaling in
    Lipid raft‐mediated Fas/CD95 apoptotic signaling in

    Type I cells (e.g., B lymphoblastoid cell line SKW6.4 and T cell lymphoma H9 cell line) are able to generate high amounts of caspase-8 that would directly activate effector caspases, thus bypassing the need for mitochondrial amplification in Fas/CD95-mediated apoptosis, whereas in contrast, type II (such as acute T cell leukemia Jurkat and CEM

  • (PDF) Rafts: a simple way to control apoptosis by
    (PDF) Rafts: a simple way to control apoptosis by

    Apoptosis Proteins of the Bcl-2 family play a pivotal role in the allows removal of abnormal growing cells and controls ho- cell-death scenario by regulating the permeabilization of the meostasis. Apoptosis may be induced by drugs, radiations, outer mitochondrial membrane

  • Host Cell Apoptosis Impairs Cryptosporidium parvum
    Host Cell Apoptosis Impairs Cryptosporidium parvum

    Host cell apoptosis may be responsible to a significant extent for the limited survival of C. parvum in vitro. Experiments aimed at reducing apoptosis and enhancing ... 1994) were purified from calf feces by salt flotation followed by sedimentation on a 15%/30% Nycodenz step gradient (Widmer et al., 1998). Oocysts were surface-sterilized in 10%

  • (PDF) Segregation of Bad from Lipid Rafts Is Implicated in
    (PDF) Segregation of Bad from Lipid Rafts Is Implicated in

    To FIGURE 2. Effect of IL-4 on association of Bad with 14-3-3 protein. analyze apoptosis, cells were diluted in ice-cold binding buffer, stained Cytoplasmic extracts from 10 ⫻ 106 IL-4-stimulated or -deprived cells with annexin and propidium iodide, and analyzed by flow cytometry

  • TRIAP1/PRELI Complexes Prevent Apoptosis ... - Cell
    TRIAP1/PRELI Complexes Prevent Apoptosis ... - Cell

    Aug 06, 2013 (A) Increased susceptibility of TRIAP1-deficient cells toward actinomycin D (ActD)-induced apoptosis. HeLa cells were transfected with scrambled (SCR) or TRIAP1-specific siRNAs and either a control plasmid (EV) or a plasmid that contains siRNA-resistant TRIAP1 encoding HA-tagged TRIAP1 (TRIAP1 HA).After induction of apoptosis with ActD (0.05 μM)

  • Human cytomegalovirus inhibits apoptosis by proteasome
    Human cytomegalovirus inhibits apoptosis by proteasome

    Mar 13, 2013 While it is not clear if Bax degradation by HPV E5 is dependent of MAM localization, together these studies suggest that ubiquitin-proteasome-mediated Bax degradation may be a common mechanism employed by viruses to prevent host cell apoptosis. HCMV infection is known to modulate the ubiquitin-proteasome system to enhance its replication

  • Exosome and Microvesicle-Enriched Fractions
    Exosome and Microvesicle-Enriched Fractions

    Based on this observation, we evaluated the biological activity on renal cell proliferation and apoptosis resistance of low (CF1), medium (CF2) and high (CF3) floatation density fractions. EVs derived from all fractions, were internalized by renal cells, CF1 and CF2 but not CF3 fraction stimulated significant cell proliferation

  • Synthetic Bichalcone TSWU-BR23 Induces Apoptosis
    Synthetic Bichalcone TSWU-BR23 Induces Apoptosis

    Using cell-surface biotinylation and sucrose density-gradient-based membrane flotation techniques, we showed that the disruption of TSWU-BR23-induced lipid raft localization of CD95/FADD by cholesterol-depleting agent (methyl-β-cyclodextrin) was

  • Identification of small extracellular vesicle subtypes
    Identification of small extracellular vesicle subtypes

    Feb 21, 2021 This study first separated sEV subtypes from follicular fluid using differential ultracentrifugation combined with iodixanol density gradient flotation and then evaluated their miRNA profile and effects on the proliferation and apoptosis of granulosa cells (GCs)

  • Two types of apoptotic cell death of rat central
    Two types of apoptotic cell death of rat central

    Two types of apoptotic cell death of rat central nervous system-derived neuroblastoma B50 and B104 cells: apoptosis induced during proliferation and after differentiation. We describe here two types of apoptotic cell death observed in the rat CNS-derived neuroblastoma B50 and B104 cells. One type was induced by dibutyryl cyclic AMP (DBcAMP) after differentiation, and the

  • Partition efficiency of cytochrome c with alcohol/salt
    Partition efficiency of cytochrome c with alcohol/salt

    Feb 01, 2020 Cytochrome c is a small water soluble spherical peripheral membrane protein with a diameter of 3.4 nm 1, 2.It is a small electron carrier in electron transport chain which is abundantly found in the intermembrane space of the mitochondria .Cytochrome c is also an important biomolecule in determining the life and death of a cell by induces the apoptosis via

  • Latin American Journal of Pharmacy
    Latin American Journal of Pharmacy

    Sodium Nitroprusside Triggered ROS Accumulation to Induce HepG2 Cells Apoptosis via AKT/Nrf2/HO-1 Pathway * CAO M., LIU L., CHEN J. & XU J. (2510) Evoxine Induces Apoptosis of Breast Cancer Cells MCF-7 via Inhibiting PI3K/AKT Pathway *

  • Apoptosis induced by oxidized low density
    Apoptosis induced by oxidized low density

    Dec 25, 2001 Apoptotic cells were identified by indirect immunofluorescence staining of the 7A6 antigen, an apoptosis-related mitochondrial membrane protein, and scored microscopically as described under Materials and methods (B,E,H)

  • Adsorption mechanism of propyl gallate as a
    Adsorption mechanism of propyl gallate as a

    Mar 15, 2019 Then the frother was added and the suspension was agitated for another 1 min before introducing air bubble to the flotation cell. After 3 min of flotation, the concentrate and tailing were collected separately, filtered, dried and weighed, based on which the recovery was calculated. 3. Results and discussion3.1

  • Selective Aggregation of Hydrophilic Gangue
    Selective Aggregation of Hydrophilic Gangue

    In a mechanical flotation cell, macro hydrodynamics is responsible for solids suspension and aggregates transport, and micro-turbulence plays an important role

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